Expression and purification of recombinant proteins from E. coli using FPLC, molecular biology techniques such as DNA digestion, ligations, cloning, midi preps, DNA agarose gel electrophoresis, PCR and tissue culture. 

University of North Carolina

Research specialist

Biochemistry

Fulltime - Research specialist

Testing the brain, bone marrow, spleen and blood samples collected from mice in the laboratory and performing various flow cytometry analysis in order to investigate the pathology of malaria.
• Analysing hematopoietic stem cell colony forming units proliferation and differentiation in malarial infection using cell culture.

Deakin University

Laboratory technician 

Houghton

Masters in Forest biotechnology

Michigan Tech University 

921 Ballarat Road, Deer Park Victoria 3023, Australia


I am Dr. Asha Lakkavaram, a graduate from Deakin University. I am looking for a suitable career in biology research where I can use my expertise. I am a temporary resident with full work rights in Australia. 
My PhD thesis is titled “Dissecting the mechanism of severe malaria”. I have studied the changes occurring in bone marrow and spleen hematopoiesis in mice with malaria, using stem cell culture and flow cytometry. I also have experience in handling bacterial cultures and aseptic techniques. 
In my previous job in biochemistry research at UNC, North Carolina, USA, I have gained hands on experience in biochemistry and molecular biology techniques such as expression and purification of recombinant proteins using FPLC, SDS PAGE electrophoresis and cloning techniques. 
I have experience working in clinical setting as well during which I have studied the combined effects of Glypizide and dietary chromium supplements on insulin levels in type II diabetes patients. I have experience working with radio immune assay which I have used to measure serum insulin levels.  
I am interested in applying for positions in biology position as they closely match my qualifications and my interests. I am a fast learner with good communication skills and feel that given a chance would prove my mettle. 



Studying combined effects of chromium and Glipizide on insulin levels in type II diabetics.
Screening and recruiting patients with type II diabetes for the study.
Estimating serum insulin levels using radio immuno assay and studying the effects of
calcium and vitamin D levels on the reversal of fluorosis.

Asha Lakkavaram / Fulltime - Research specialist

Research specialist

University of North Carolina

Progress

Skills